| Schauspieler | Rolle | Synchronsprecher | ||
| Margot Robbie Margot Robbie | Harley Quinn | Anne Helm Anne Helm | ||
| Ewan McGregor Ewan McGregor | Roman Sionis / Black Mask | Philipp Moog Philipp Moog | ||
| Paloma Rabinov | Bitchy Teammate | Magdalena Helmig | ||
| Steven Williams | Captain Patrick Erickson | Roland Hemmo Roland Hemmo | ||
| Ella Jay Basco | Cassandra Cain | Derya Akyol Derya Akyol | ||
| Jurnee Smollett | Dinah Lance / Black Canary | Anja Stadlober | ||
| Dana Lee | Doc | Fang Y. Fang Y. | ||
| Ali Wong | Ellen Yee | Uschi Hugo Uschi Hugo | ||
| Bojana Novakovic | Erika | Marieke Oeffinger Marieke Oeffinger | ||
| Ryan Watson | Gefangener #1 | Samuel Zekarias | ||
| Mary Elizabeth Winstead Mary Elizabeth Winstead | Helena Bertinelli / Huntress | Maria Koschny Maria Koschny | ||
| Derek Wilson | Monroe | Jaron Löwenberg Jaron Löwenberg | ||
| François Chau | Mr. Keo | Matthias Klages Matthias Klages | ||
| Michael Masini | Officer Drago | Samuel Zekarias | ||
| Rosie Perez | Renee Montoya | Claudia Urbschat Mingues Claudia Urbschat Mingues | ||
| Zack Whyel | Romans Heiler | Mario Klischies | ||
| Daniel Bernhardt | Sionis' Chauffeur | Marcus Off Marcus Off | ||
| Shahaub Roudbari | Smarmy Guy | Paul Matzke | ||
| Chris Messina | Victor Zsasz | Asad Schwarz Asad Schwarz | ||
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